Real-time two-photon confocal microscopy using a femtosecond, amplified Ti:sapphire system.

نویسندگان

  • G J Brakenhoff
  • J Squier
  • T Norris
  • A C Bliton
  • M H Wade
  • B Athey
چکیده

The bilateral imaging approach known from confocal applications operating in the line mode was used to realize real-time two-photon imaging. It is shown that the sectioning inherent to two-photon imaging could be improved by the introduction of a confocal line aperture in the imaging path. Using a high-power, low-repetition-rate amplified Ti:sapphire system, various biological objects were visualized including live boar sperm.

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عنوان ژورنال:
  • Journal of microscopy

دوره 181 Pt 3  شماره 

صفحات  -

تاریخ انتشار 1996